Sub-chronic effect of metal working fluids on pulmonary functions in B6C3F1 mice: role of vitamin E.
Kommineni-C; Kisin-E; Murray-A; Smith-CA; Goldsmith-T; Reynolds-JS; Castranova-V; Frazer-DG; Shvedova-AA
Toxicologist 2002 Mar; 66(1-S):89-90
Metal working fluids (MWFs) are widely used in many industries. Exposures to MWFs are known to cause a spectrum of respiratory effects like excessive cough and attendant secretary products, bronchitis and physical discomfort as evidenced by tightness of chest. This lead us to examine if an inhalation exposure to a semi-synthetic neat MWF produces any morphological as well as functional alterations in the lungs of mice. Morphological/functional and antioxidant outcomes in lungs were studied after inhalation exposure to MWF of vitamin E deficient and sufficient mice. Mice were given vitamin E deficient or basal diets for 35 weeks. Inhalation exposure to MWF started after 18 weeks on respective diets. Mice were exposed to MWF for 85 individual exposures (17 weeks, five days a week, 6 hours a day). No morphological alterations were seen in the lungs of mice from the studied groups. However, functional deficits in breathing rate, peak inspiratory/expiratory flow, minute ventilation, and tidal volume, along with marked reduction in the lung levels of GSH, protein thiols and ascorbate were in evidence in mice maintained on vitamin E deficient diet. Mice maintained on sufficient diet and exposed to MWF had decreased tidal volume compared to respective controls. Exposure to MWF resulted in accumulation of peroxidative products, and reduction of ascorbate content in the lungs compared to vitamin E sufficient or deficient controls. The results of this study indicate that dietary vitamin E deficiency enhanced subchronic effect of inhalation exposure to MWF.
Metalworking; Pulmonary-function-tests; Respiratory-function-tests; Bronchial-asthma; Animal-studies; Vitamins; Laboratory-animals; Pulmonary-system-disorders; Respiratory-system-disorders; Metalworking-fluids
The Toxicologist. Society of Toxicology 41st Annual Meeting and ToxExpo, March 17-21, 2002, Nashville, Tennessee