Assessment of DNA strand breaks in leukocytes of workers occupationally exposed to 1-bromopropane.
Toraason-M; Lynch-DW; DeBord-DG; Singh-N; Nemhauser-J
Toxicologist 2003 Mar; 72(S-1):250
As part of two NIOSH Health Hazard Evaluations, the comet assay was performed on peripheral leukocytes from workers to determine if occupational exposure to 1- bromprompane (1-BP) induced DNA strand breaks. Start-of- and end-of-workweek blood and urine samples were collected from 41 and 22 workers at 2 facilities where 1-BP was used as a solvent for spray adhesives in furniture manufacturing. Exposure to 1-BP was assessed from personal breathing zone samples collected for 1-3 days up to 8 hrs per day for calculation of 8 hr time waited average (TWA) 1-BP concentrations. Bromide was measured in blood and urine as an internal biomarker of exposure. Overall, 1-BP TWA concentrations ranged from 0.2 - 271 ppm at site 1 and from 4 - 42.7 ppm at site 2. The highest exposures were in workers classified as sprayers. 1-BP TWA concentrations were statistically significantly correlated with blood and urine bromide concentrations. For estimation of DNA damage, comets were produced by alkaline microgel-electrophoresis and analyzed using VisComet image analysis software. One hundred leukocytes from each blood sample (stored at -80 oC until analysis) were evaluated for comet extent, tail extent, tail moment, and tail integrated intensity among other endpoints as a indexes of DNA single strand breaks/alkali labile sites. Start-of- and end-of-workweek comet endpoints were compared with environmental and internal exposure indices using linear regression analysis. Comet results were also stratified based on job classification. Preliminary analyses indicate that occupational exposures to 1-BP at these two facilities were not associated with the level of DNA strand breaks in leukocytes of exposed workers.
Leukocytes; Occupational-exposure; Workers; Health-hazards; Blood-samples; Urinalysis; Breathing-zone; Environmental-exposure; Mutagenicity; Solvents
Research Tools and Approaches: Exposure Assessment Methods
The Toxicologist. Society of Toxicology 42nd Annual Meeting and ToxExpo, Cutting-Edge Science, Networking, New Perspectives, March 9-13, 2003, Salt Lake City, Utah