Silica-induced toxicity: in vitro and in vivo protective effects of taurine.
Vallyathan-V; Pack-D; Patel-S
Toxicologist 2003 Mar; 72(S-1):45-46
Taurine has been suggested to have cytoprotective actions through different mechanisms including antioxidant effects. Taurine has been proposed to be a membrane stabilizer thereby preventing oxidative damage. The aim of the present studies was to test whether taurine might act to attenuate silica-induced toxicity in vitro and in vivo. The presence of taurine resulted in a reduction of cytotoxicity with alveolar macrophages exposed to crystalline silica. To investigate further whether taurine would function effectively in the prevention of in vivo toxicity from exposure to silica we exposed rats by intratracheal instillation to a single dose of 5 mg silica coated with 100 mM taurine and compared the pulmonary response to rats exposed to silica or vehicle saline. Rats were sacrificed 1, 3, or 7 days postexposure, and lungs were lavaged to monitor inflammatory cells (alveolar macrophages, neutrophils, lymphocytes, eosinophils), leakage of albumin, protein and enzymes (LDH, NAG), and chemiluminescence as markers of inflammation, cytotoxicity and reactive oxygen species generated, respectively. Surface coating and coexposure of silica with taurine significantly decreased silica-induced lung injury. In conclusion, taurine showed beneficial effects in both in vitro and in vivo models of silica toxicity. This result confirms previous studies obtained in other models of lung injury.
In-vivo-studies; In-vitro-studies; Cytotoxic-effects; Laboratory-animals; Respiratory-system-disorders; Pulmonary-system-disorders; Lung-disease
Work Environment and Workforce: Mixed Exposures
The Toxicologist. Society of Toxicology 42nd Annual Meeting and ToxExpo, Cutting-Edge Science, Networking, New Perspectives, March 9-13, 2003, Salt Lake City, Utah