Interaction between primary alveolar macrophages (AM) and primary alveolar type II (TII) cells under basal conditions and after lipopolysaccharide (LPS) or quartz exposure.
Kanj-RS; Kang-JL; Castranova-V
Toxicologist 2003 Mar; 72(S-1):45
This study evaluated the mutual interactions between AM and TII cells under unstimulated or LPS (10 microg/ml) or quartz (100 microg/ml) exposure conditions. AM were obtained by bronchoalveolar lavage of rats, while rat TII cells were isolated by enzymatic digestion and purified by panning (approximately 20 million cells/rat, 95% pure). AM and TII cells were co-cultured either separated by transwell inserts or in a single well to allow physical contact. After an 18 hour culture period in the absence or presence of stimulant, the medium was assayed for tumor necrosis factor-alpha (TNF-alpha), macrophage inflammatory protein-2 (MIP-2), interleukin-6 (IL-6), interleukin-1 beta (IL-1Beta) and nitric oxide (NO). Cell viability, which was measured as lactate dehydrogenase (LDH) released from the cells into the medium, was not affected by either transwell or contact co-culture under basal or stimulated conditions. Under basal conditions, co-culture of AM and TII cells in transwells significantly potentiated the release of TNF-alpha, MIP-2, IL-6 and NO above the sum of the production by these cells cultured separately. Physical contact between AM and TII cells mitigated this potentiation, which was further decreased by exposure to LPS or quartz. Indeed, under stimulated conditions, physical contact actually decreased the production of some of these inflammatory products below the sum of the production by these cells cultured separately. These results indicate that cross-talk between AM and TII cells is complex. It appears to vary with the distance and/or contact between the two cell types, and with exposure to different stimulants.
Laboratory-animals; Respiratory-system-disorders; Pulmonary-system-disorders; Quartz-dust; Silica-dusts; Lung-disease; Lung-fibrosis; Man-made-mineral-fibers
The Toxicologist. Society of Toxicology 42nd Annual Meeting and ToxExpo, Cutting-Edge Science, Networking, New Perspectives, March 9-13, 2003, Salt Lake City, Utah