Biomarkers of human glioma cell exposure to electromagnetic fields.
Hennessey-EM; U'Ren-LW; Savage-RE; Kanitz-MH; Lotz-WG; Hanneman-WH
Toxicologist 2003 Mar; 72(S-1):13-14
Several recent reviews on the potential health effects of electric and magnetic fields (EMFs) have concluded that power frequency EMF exposures are potentially carcinogenic to humans. Similarly, recent pooled analyses examining the relationship between magnetic fields (MF) exposure and childhood cancer indicate that exposure to residential MF exceeding 3 or 4 milligauss (mG) are associated with a 70-100% increased risk of acute lymphocytic leukemia, the most common form of cancer in children. In light of these data, the National Institute for Occupational Safety and Health (NIOSH), together with Colorado State University (CSU) are investigating the effects of putative occupational carcinogens on cancer-linked molecular events in appropriate cellular systems. We are currently interested in the biological plausibility of EMFs as a putative carcinogen in SF767 human glioma cells. Here we study the effect of short-term environmental-level EMF exposure using cDNA microarrays. Following a 3 hr exposure we investigate changes in gene induction to determine appropriate genetic biomarkers of effect of exposure to EMF. At 70% confluence SF767 are exposed in the presence or absence of either epidermal growth factor (EGF) or melatonin, to a 12 mG, 60 Hz EMF. Ambient field strength in the cell culture incubator is maintained at 2 mG, 60 Hz. Total RNA was then isolated for use on the PerkinElmer oncogene array. Computer analysis and comparison of gene up and down regulation is performed across groups. These data suggest a limited yet complicated response of glioma cells to environmental field exposure. The number of oncogenes induced by EMF alone and in the presence or absence of melatonin and/or EGF range from 8 to over 40. The number of suppressed oncogenes ranges from 7 to more than 20. Current cluster analysis of this data may offer preliminary evidence of the possible role of EMF as a carcinogen in human glioma cells.
Electromagnetic-energy; Electromagnetic-fields; Electromagnetic-radiation; Carcinogens; Carcinogenesis; Magnetic-fields; Age-factors; Sociological-factors; Blood-disorders
The Toxicologist. Society of Toxicology 42nd Annual Meeting and ToxExpo, Cutting-Edge Science, Networking, New Perspectives, March 9-13, 2003, Salt Lake City, Utah