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Validation of a field-practical collection method for bromopropane in exhaled breath.

Hanley K; Sanderson W; Booher D
American Industrial Hygiene Conference and Exposition, June 1-6, 2002, San Diego, California. Fairfax, VA: American Industrial Hygiene Association, 2002 Jun; :85
1-Bromopropane (1-BP) has been marketed as an alternative for ozone depleting solvents and suspect carcinogens and is used for metal, precision, and electronics cleaning; aerosols; and adhesives. Toxicity of 1-BP is poorly understood but it may be a neurologic, reproductive and hematologic toxin. Sparse exposure information has prompted NIOSH to conduct a multi-industry exposure assessment using inhalation, exhaled breath, and urinary metabolite measurements. Exhaled breath analysis can be a powerful, non-invasive tool that indirectly measures inhalation and dermal exposure. However, validated analytical methods specifically for 1-BP in exhaled breath are unavailable. The objective of this study was to evaluate an exhaled breath method for 1-BP analysis. The method must be accurate, sensitive, and unaffected by water vapor to measure trace levels consistently without contamination or deterioration. Three liter Tedlar breath bags were used which contained waste air diverting valves to ensure end-tidal breath collection. Bags were filled with nitrogen or breath and spiked, in triplicate, with nominal concentrations of 0.2; 0.5; 2; and 5 ppm. The bag mixture was then collected on Anasorb CMS synthetic charcoal tubes at a pump flow rate of 100 cc/min and analyzed by gas chromatography via NIOSH Method 1025. The LOD was 7 ug, therefore, 1-BP should be detected as low as 0.05 ppm in 3 liters. All bag samples detected the analyte, some as low as 2 ug. The mean recovery of 1-BP with this method was 0.72 and 0.73 for nitrogen and breath mixtures, respectively. Stability of BP in breath was evaluated by sampling 0.5 and 5.0 ppm bag concentrations after 3 hours and the mean recovery was nearly the same (0.735). This sampling strategy allows breath samples to be analyzed by a contract laboratory, thereby eliminating chemical analysis with portable instrumentation in field surveys.
Breathing; Solvents; Carcinogens; Aerosols; Adhesives; Toxins; Inhalants; Analytical-methods; Sampling; Solvent-vapors
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American Industrial Hygiene Conference and Exposition, June 1-6, 2002, San Diego, California
Page last reviewed: September 2, 2020
Content source: National Institute for Occupational Safety and Health Education and Information Division