Determination of unique microbial volatile organic compounds produced by five Aspergillus species commonly found in problem buildings.
Gao-P; Korley-F; Martin-J; Chen-BT
Am Ind Hyg Assoc J 2002 Mar/Apr; 63(2):135-140
This study identified unique microbial volatile organic compounds (UMVOCs) produced by five Aspergillus species (A. fumigatus, A. versicolor, A. sydowi, A. flavus, and A. niger) cultivated on malt extract agar and gypsum board. The hypothesis was that UMVOCs can be used to predict the presence of Aspergillus species. During the cultivation humidified air was continually supplied and evenly distributed through each of the culture flasks. Volatile metabolites were collected using Tenax TA tubes on Days 8, 16, and 30 after inoculation. The volatile metabolites were determined by gas chromatography/mass spectroscopy after thermal desorption. Nine compounds recognized as UMVOCs--3-methyl-1-butanol; 2-methyl-1-propanol; terpineol; 2-heptanone; 1-octen-3-ol; dimethyl disulfide; 2-hexanone; 3-octanone; and 2-pentylfuran--were found on the cultures in detectable amounts. The first two compounds were detected at the highest frequency when combining both media. The first four compounds were found to be the dominant UMVOCs on gypsum board, which could be used as chemical markers of the common Aspergillus species grown indoors.
Indoor-air-pollution; Air-quality; Air-contamination; Odors; Fungi; Metabolites; Humidity; Organic-chemicals; Microorganisms; Indoor-environmental-quality;
Author Keywords: Aspergillus; cultures; fungal contamination; indoor air quality; problem buildings; unique MVOCs
Pengfei Gao, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, WV 26505
Research Tools and Approaches: Exposure Assessment Methods
American Industrial Hygiene Association Journal