NIOSHTIC-2 Publications Search

Protective roles of NF-kB for chromium (VI)-induced cytotoxicity is revealed by expression of IkB kinase-B mutant.

Chen F; Bower J; Leonard S; Ding M; Lu Y; Rojanasakul Y; Kung H; Vallyathan V; Castranova V; Shi X

J Biol Chem 2002 Feb; 277(5):3342-3349

https://doi.org/10.1074/jbc.M101089200

20022295

To delineate the molecular mechanisms of NF-B-mediated regulation of chromium(VI)-induced cell death, the signaling pathway leading to the activation of NF-B was interrupted by stable transfection of a kinase-mutated form of IB kinase (IKK-KM). Here we demonstrate a novel role for the NF-B transcription factor in inhibiting chromium(VI)-induced cell death. Inhibition of NF-B by IKK-KM or IKK gene deficiency resulted in a spontaneous cleavage of Bcl-xl antiapoptotic protein due to the elevated caspase-3 activity. DNA microarray assay suggested a decreased expression of genes encoding antiapoptotic proteins, cIAP1 and cIAP2, in the cells overexpressing IKK-KM. Chromium(VI) treatment of these NF-B-inhibited cells induced necrotic-like cell death. Such chromium(VI)-induced cell killing could be partially inhibited by expression of exogenous cIAP1, an inhibitor of caspases, indicating that caspases along with others may be involved in chromium(VI)-induced cell death. These results suggest that NF-B is essential for inhibiting toxic metal-induced cytotoxicity. Such inhibition may involve up-regulation of the expression of anti-death proteins including cIAP1 that prevents spontaneous caspase activation and subsequent cleavage of Bcl-xl protein.

Chromium-compounds; Cytotoxicity; Carcinogenesis; Humans; Toxic-effects

PPRB of NIOSH, 1095 Willowdale Rd., Morgantown, WV 26505

JBCHA3

7440-47-3; 18540-29-9

20020201

Journal Article

xshi@cdc.gov

Interagency Agreement

2002

IA-98-18-00-M2

5

0021-9258

HELD

Research Tools and Approaches: Cancer Research Methods

Journal of Biological Chemistry

WV