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Cytokine and nitric oxide release by J774A.1 macrophages is not regulated by estradiol.

Authors
Woodfork-KA; Schuller-KC; Huffman-LJ
Source
Life Sci 2001 Sep; 69(19):2287-2294
NIOSHTIC No.
20021930
Abstract
Estradiol is able to regulate the release of inflammatory mediators by macrophages; however, the presence, extent, and direction of this modulation varies with species, tissue of origin, and cell culture conditions. This study examines the effects of 17-beta-estradiol (E2) on the release of inflammatory mediators by the J774A.1 mouse macrophage cell line. For experiments, cells were plated in phenol red-free DMEM containing 5% charcoal-dextran stripped calf serum. Western analysis showed that J774A.1 cells contain the estrogen receptor alpha (ER alpha) protein. We found that physiological and pharmacological levels of E2 (10(-12) M-10(-6) M) have no effect on the release of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), or monocyte chemoattractant protein-1 (MCP-1). This suggests that J774A.1 cells grown under these culture conditions would be useful for the investigation of non-estrogen-dependent mechanisms by which certain endocrine disruptors may affect their targets in macrophages.
Keywords
Cell-cultures; Oxides; Endocrine-system; Physiological-effects; Cell-biology; Cell-function; Cellular-function; Cellular-reactions; In-vitro-study; Author Keywords: Macrophage; Estrogen; Nitric oxide; Tumor necrosis factor-a; Interleukin-6; Monocyte chemoattractant protein-1; Endocrine disruptors
Contact
Karen A. Woodfork, Department of Biochemistry and Molecular Pharmacology, Robert C. Byrd Health Sciences Center, West Virginia University, P.O. Box 9142, Morgantown, WV 26506
CODEN
LIFSAK
CAS No.
10102-43-9
Publication Date
20010928
Document Type
Journal Article
Email Address
kwoodfork@hsc.wvu.edu
Fiscal Year
2001
NTIS Accession No.
NTIS Price
Issue of Publication
19
ISSN
0024-3205
NIOSH Division
HELD
Source Name
Life Sciences
State
WV
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