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Cytokine and nitric oxide release by J774A.1 macrophages is not regulated by estradiol.
Woodfork KA; Schuller KC; Huffman LJ
Life Sci 2001 Sep; 69(19):2287-2294
Estradiol is able to regulate the release of inflammatory mediators by macrophages; however, the presence, extent, and direction of this modulation varies with species, tissue of origin, and cell culture conditions. This study examines the effects of 17-beta-estradiol (E2) on the release of inflammatory mediators by the J774A.1 mouse macrophage cell line. For experiments, cells were plated in phenol red-free DMEM containing 5% charcoal-dextran stripped calf serum. Western analysis showed that J774A.1 cells contain the estrogen receptor alpha (ER alpha) protein. We found that physiological and pharmacological levels of E2 (10(-12) M-10(-6) M) have no effect on the release of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), or monocyte chemoattractant protein-1 (MCP-1). This suggests that J774A.1 cells grown under these culture conditions would be useful for the investigation of non-estrogen-dependent mechanisms by which certain endocrine disruptors may affect their targets in macrophages.
Cell-cultures; Oxides; Endocrine-system; Physiological-effects; Cell-biology; Cell-function; Cellular-function; Cellular-reactions; In-vitro-study; Author Keywords: Macrophage; Estrogen; Nitric oxide; Tumor necrosis factor-a; Interleukin-6; Monocyte chemoattractant protein-1; Endocrine disruptors
Karen A. Woodfork, Department of Biochemistry and Molecular Pharmacology, Robert C. Byrd Health Sciences Center, West Virginia University, P.O. Box 9142, Morgantown, WV 26506
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