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Quantitative analysis of phospholipid peroxidation and antioxidant protection in live human epidermal keratinocytes.

Authors
Shvedova AA; Tyurina YY; Tyurin VA; Kikuchi Y; Kagan VE; Quinn PJ
Source
Biosci Rep 2001 Feb; 21(1):33-43
NIOSHTIC No.
20021872
Abstract
To characterize oxidative stress in phospholipids of normal human epidermal keratinocytes we metabolically labeled their membrane phospholipids with a natural oxidation-sensitive fluorescent fatty acid, cis-parinaric acid, and exposed the cells to two different sources of oxidants--a lipid-soluble azo-initiator of peroxyl radicals, 2,2'-azobis(2,4-dimethyl-valeronitrile), AMVN, and a superoxide generator, xanthine oxidase/xanthine. We demonstrated that both oxidants induced pronounced oxidation of four major classes of cis-parinaric acid-labeled phospholipids-phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol-in normal human epidermal keratinocytes that was not detectable as any significant change of their phospholipid composition. Vitamin E was effective in protecting the cells against phospholipid peroxidation. Since viability of normal human epidermal keratinocytes was not changed either by labeling or exposure to oxidants the labeling protocol and oxidative stress employed are compatible with the quantitative analysis of phospholipid peroxidation in viable cells.
Keywords
Antioxidants; Phospholipids; Stress; Exposure-levels; Skin; Carcinogenesis; Author Keywords: keratinocytes; phospholipid peroxidation; oxidative stress; xanthine; xanthine oxidase; AMVN; alpha-tocopherol
Contact
Anna A. Shvedova, Health Effects Laboratory Division, Pathology and Physiology Research Branch, NIOSH, 1095 Willowdale Road, Morgantown, WV 26505
CODEN
BRPTDT
Publication Date
20010201
Document Type
Journal Article
Editors
Pasternak CA; Lennarz WJ
Fiscal Year
2001
Issue of Publication
1
ISSN
0144-8463
NIOSH Division
HELD
Source Name
Bioscience Reports
State
WV; PA
Page last reviewed: December 4, 2020
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