Used semi-synthetic metal working fluid (UMWF): dermal and systemic effects in B6C3F1 mice.
Kommineni-C; Shvedova-AA; Al-Humadi-NH
Toxicologist 2001 Mar; 60(1):59
In our previous studies we demonstrated that dermal exposure to unused MWF caused a significant increase in skin histamine, mast cell numbers, malondialdehyde (MDA), and alcohol dehydrogenase (AD and reduction in ascorbic acid (AA) levels. In use, MWF becomes contaminated with microorganisms (e.g., gram negative bacteria) and their products (endotoxin) which may alter the toxicity of these fluids. To complement the previous studies, a sample of MWF used for 11 months and contained approximately 5000 endotoxin units (EU)/ml was tested. Unshaven mice of both sexes at 6 weeks of age were topically given 200il of used MWF 2 days a week for 6 weeks. Total skin histamine (both sexes), testicular and liver AA (both sexes), and MDA (female liver) were reduced significantly (P < 0.05) compared to untreated control groups. Similar reductions of AA and MDA were observed when mice were topically exposed to endotoxin alone at 5000EU/ml. The AD levels in the male (testes and liver) were similar to those in controls; however, the AD levels in the treated female livers were increased when the mice were exposed to used MWF or to 5000 EU/ml of endotoxin alone. These results suggest that the toxic effects of used MWF were different than those observed with unused MWF and that differences were largely due to the endotoxin contamination of the MWF.
Metals; Metalworking; Animal-studies; Laboratory-animals; Exposure-levels; Endotoxins; Microorganisms
The Toxicologist. Society of Toxicology 40th Annual Meeting, March 25-29, 2001, San Francisco, California