An in vivo animal model for the investigation of acute and chronic skeletal muscle injury.
Authors
Cutlip RG; Wirth O; Miller R; Geronilla K; Mowrey K
Source
Advances in Occupational Ergonomics and Safety: XVth Annual Conference of the International Society for Occupational Ergonomics and Safety, Fairfax, Virgina, June 4 - 7, 2001. AC Bittner Jr, PC Champney, SJ Morrissey, eds. Washington, DC: IOS Press, 2001 Jan; 4:394-401
Link
NIOSHTIC No.
20021283
Abstract
An in vivo model to study skeletal muscle injury is described. A computer-controlled custom-designed rat dynamometer is used to control biomechanical inputs such as range of motion, velocity, acceleration, and number of repetitions to study skeletal muscle injury in rats. Anesthetized rats are placed supine in the dynamometer and the left foot placed in a load cell with the ankle axis aligned with the axis of rotation of the motor. Platinum electrodes are placed subcutaneously to branch either the peroneal nerve (to activate the dorsi flexor muscles of the hind limb) or the tibial nerve (to activate the plantar flexor muscles of the hind limb). The free ends of the wire electrodes are connected to a computer-controlled nerve stimulator. The dynamometer can be programmed to produced controlled angular movement about the ankle axis to generate isometric, concentric, and reciprocal concentric/eccentric muscle actions of either the plantar flexor or dorsi flexor muscles. This model has distinct advantages as compared to invasive in vitro or in situ preparations of isolated muscles or muscle fibers. Muscle response and injury can be studied in a more physiologically representative fashion with the neural and vascular supplies and muscle-tendon complexes intact. The non-invasive features of this model are well suited for the study of chronic muscle injury. When the biomechanical data from the dynamometer are combined with histological and biochemical analyses of muscle tissue, this model can provide comprehensive data for studying acute and chronic skeletal muscle pathomechanics.
Keywords
In vivo studies; Laboratory animals; Animals; Animal studies; Models; Skeletal system disorders; Musculoskeletal system disorders; Injuries; Muscle tissue
Publication Date
20010101
Document Type
Conference/Symposia Proceedings
Editors
Bittner AC Jr.; Champney PC; Morrissey SJ
Fiscal Year
2001
ISBN No.
9781586031749
ISSN
1384-2269
NIOSH Division
HELD
Source Name
Advances in Occupational Ergonomics and Safety: XVth Annual Conference of the International Society for Occupational Ergonomics and Safety, Fairfax, Virgina, June 4 - 7, 2001
Links with this icon indicate that you are leaving the CDC website.
The Centers for Disease Control and Prevention (CDC) cannot attest to the accuracy of a non-federal website.
Linking to a non-federal website does not constitute an endorsement by CDC or any of its employees of the sponsors or the information and products presented on the website.
You will be subject to the destination website's privacy policy when you follow the link.
CDC is not responsible for Section 508 compliance (accessibility) on other federal or private website.
For more information on CDC's web notification policies, see Website Disclaimers.
CDC.gov Privacy Settings
We take your privacy seriously. You can review and change the way we collect information below.
These cookies allow us to count visits and traffic sources so we can measure and improve the performance of our site. They help us to know which pages are the most and least popular and see how visitors move around the site. All information these cookies collect is aggregated and therefore anonymous. If you do not allow these cookies we will not know when you have visited our site, and will not be able to monitor its performance.
Cookies used to make website functionality more relevant to you. These cookies perform functions like remembering presentation options or choices and, in some cases, delivery of web content that based on self-identified area of interests.
Cookies used to track the effectiveness of CDC public health campaigns through clickthrough data.
Cookies used to enable you to share pages and content that you find interesting on CDC.gov through third party social networking and other websites. These cookies may also be used for advertising purposes by these third parties.
Thank you for taking the time to confirm your preferences. If you need to go back and make any changes, you can always do so by going to our Privacy Policy page.