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Treatment with corticosterone suppresses the early induction of MCP-1 after neuronal injury but does not prevent gliosis.
Little AR; O'Callaghan JP
Abstr - Soc Neurosci 2000 Nov; 26:1944
Cytokines are proposed to play a role in central nervous system responses to injury and disease. One such response is neuronal damage-induced glial activation (gliosis). Here, we used the neurotoxic organometal, trimethyltin (TMT), to damage hippocampal pyramidal cells and elicit a glial response. In contrast to ischemic or traumatic brain injury models, TMT-induced neuronal damage is not associated with a compromised blood-brain barrier, nor is it associated with an inflammatory response, as evidenced by a lack of induction in TNF-alpha or IL-1B. Other chemokines and cytokines however, have been implicated in neural degeneration/regeneration responses and in the induction of gliosis. We confirmed the injury-associated expression of the chemokine monocyte chemoattractant protein (MCP)-1, in the TMT injury model and evaluated the effects of immune suppression on MCP-1 in relation to injury-induced gliosis. Induction of MCP-1 mRNA was assessed using semi-quantitative and quantitative RT-PCR (Taqman) at 2,5,& 8 hrs and 1,2,3,5,7 & 21 days after TMT (8 mg/kg, i.p.). Corticosterone (CORT) was administered in the drinking water (200 microg/ml). MCP-1 was elevated 100-fold 5 hours post TMT and increased until day 5. GFAP mRNA did not begin to increase until day 3, followed by an increase in GFAP (protein) at day 5. MCP-1 remained at control levels at all time points in the CORT/TMT group. These results demonstrate an involvement of MCP-1 in neural injury. MCP-1 and other inflammatory mediators do not appear to be required for glial activation because suppression of pro-inflammatory cytokines and chemokines by CORT does not alter the injury-induced glial response.
Central nervous system; Central nervous system disorders; Animal studies; Animals; Laboratory animals; Neurotoxic effects; Neurotoxins; Neurological system; Brain damage; Models; Exposure levels; Cellular reactions
Abstract; Conference/Symposia Proceedings
Abstracts - Society for Neuroscience. Society for Neuroscience's 30th Annual Meeting, New Orleans, LA, November 4 - 9, 2000
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