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Elevated oxidative stress in skin of B6C3F1 mice affects dermal exposure to machine working fluid.

Kommineni CV; Kisin ER; Al-Humadi N; Castranova V; Shvedova AA
Toxicol Ind Health 2000; 16(7-8):267-276
Metal working fluids (MWFs) are widely used in industry for metal cutting, drilling, shaping, lubricating, and milling. Potential for dermal exposure to MWFs exists for a large number of men and women via aerosols and splashing during the machining operations. It has been reported earlier that occupational exposure to MWFs causes allergic and irritant contact dermatitis. Previously, we showed that dermal exposure of female and male B6C3F1 mice to 5% MWFs for 3 months resulted in accumulation of mast cells and elevation of histamine in the skin. Topical exposure to MWF also resulted in elevated oxidative stress in the liver of both sexes and the testes in males. The goal of this study was to evaluate the interaction between oxidative stress in the skin and topical application of MWF. Oxidative stress in skin of B6C3F1 mice of both sexes was generated by intradermal injection of the hydrogen peroxide (H2O2) -producing enzyme, glucose oxidase with polyethylene glycol (GOD+PEG). In mice given GOD+PEG, topical treatment with MWF (200 microl, 30%, for 1, 3, or 7 days) resulted in a mixed inflammatory cell response, accumulation of peroxidative products, and reduction of GSH content in the skin. Such changes were not observed with MWF treatment alone. These data indicate that oxidative stress can enhance dermal inflammation caused by occupational exposure to MWF.
Stress; Skin; Metals; Exposure levels; Aerosols; Occupational exposure; Metalworking fluids; Metalworking; Metalworking industry; Inhalation studies; Workers; Machinists; Machine operators; Demographic characteristics; Sex factors; Health hazards; Allergic dermatitis; Contact dermatitis; Dermatitis; Laboratory animals; Animals; Animal studies; Skin irritants; Author Keywords: glucose oxidase; GSH; inflammation; metal working fluid; oxidative stress; skin
Dr. Anna A. Shvedova, Health Effects Laboratory Division, Pathology and Physiology Research Branch, NIOSH m/s 2015, 1095 Willowdale Road, Morgantown , WV 26505
Publication Date
Document Type
Journal Article
Email Address
Mehlman MA
Fiscal Year
Issue of Publication
NIOSH Division
Priority Area
Research Tools and Approaches; Exposure Assessment Methods
Source Name
Toxicology and Industrial Health
Page last reviewed: April 9, 2021
Content source: National Institute for Occupational Safety and Health Education and Information Division