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The effect of restraint on the development of allergic contact dermatitis is dependent upon the timing of the restraint.

Flint MS; Tinkle SS
J Invest Dermatol 2000 May; 114(4):188
Previous studies have shown that restraint stress modifies the development of allergic contact dermatitis (ACD). We hypothesized that the timing of restraint with respect to the chemical application influences restraint-induced modulation of ACD. Male BALB/c and C57BL/6 mice were sensitized on the flank on days I and 2 with 0.5% di-nitrofluorobenzene (DNFB) and challenged on the ear on day 6 with 0.25% DNFB. Mice were restrained for 2 hours prior to chemical application on day I only, on days 1 and 2, or on day 6 only. We assessed serum corticosterone to verify activation of the hypothalamic-pituitary-adrenal axis and ear swelling as a measure of the inflammatory/immune response. Restraint increased serum corticosterone 10 16 fold in both strains of mice. DNFB stimulated significant ear swelling in non-restrained mice. The ear swelling response was suppressed in DNFB-treated mice restrained for 2 hours on day 1 only, but was unchanged in DNFB-treated mice restrained on days 1 and 2. In contrast, mice restrained only on day 6 displayed significantly enhanced ear swelling, approximately 20% higher than levels measured in DNFB-treated, non-restrained mice. Administration of the glucocorticoid receptor antagonist, RU486, had little effect on the suppressed ear swelling response but did block the day 6 restraint-induced increase. These data demonstrate that timing of restraint affects the type and degree of modulation of chemical-induced ear swelling and that corticosterone may be more important in the enhanced ear swelling response than in the suppression of ear swelling.
Contact dermatitis; Contact allergies; Allergic dermatitis; Laboratory animals; Animals; Animal studies; Immune reaction
Publication Date
Document Type
Abstract; Conference/Symposia Proceedings
Fiscal Year
Issue of Publication
NIOSH Division
Source Name
Journal of Investigative Dermatology
Page last reviewed: April 9, 2021
Content source: National Institute for Occupational Safety and Health Education and Information Division