The effect of vibration on endothelin-1 production by dermal microvascular endothelial cells.
Ann Biomed Eng 2000 Oct; 28(1):T6.141
Hand-arm vibration syndrome (HAVS) is a disorder affecting workers using vibrating hand tools such as chainsaws, pneumatic hammers and drills, and grinders. Vascular disorders are most commonly associated with HAVS and manifest as episodes of blanching of the fingers, especially in response to cold. Because of the similarity of symptoms of HAVS and Raynaud's phenomenon, HAVS is also referred to as Raynaud's phenomenon of occupational origin or vibration white finger. The purpose of this study is to develop a cellular model to investigate the effects of vibration on vascular cells. We grew human dermal microvascular endothelial cells on gelatin-coated 35 mm culture dishes and exposed them to a 125 Hz mechanical vibration at an acceleration of 10 m/s2. After 4 hours, supernatants were collected from vibrated cells and from stationary controls. Endothelin-1 (ET-1) protein levels in the supernatant were measured using an enzyme immunoassay. The number of cells in each dish was counted and used to normalize ET-1 concentrations. Preliminary results indicate that ET-1 concentrations were higher for vibrated cells as compared to controls. Our results suggest that vibration-induced alterations in ET-1 production may be involved in the etiology of HAVS.
Vibration-effects; Hand-tools; Hand-injuries; Vibration-exposure; Workers; Chain-saws; Arm-injuries; Etiology; Models
Abstract; Conference/Symposia Proceedings
Annals of Biomedical Engineering. Biomedical Engineering Society 2000 Annual Fall Meeting 12-14 October 2000