Use of fluorescence microbead immunosorbent assays (FMIAs) for pesticide biomonitoring.
Biagini R; Striley C; Murphy D; MacKenzie B; Robertson S
Pacifichem 2000, Proceedings of the 2000 International Chemical Congress of Pacific Basin Societies, December 14-19, 2000, Honolulu, Hawaii. Washington, DC: American Chemical Society, 2000 Dec; (Pt 1):AGRO-263
Quantitative analyses for urinary excreted pesticides/pesticide metabolites are usually performed using classical chemical/instrumental analysis techniques. These procedures may be costly, time consuming, and labor intensive. Alternatives to instrumental analyses are enzyme immunoassays (EIA), where analyses can be quantitated by using antibodies directed against urinary pesticides/metabolites. EIAs have the benefit of being inexpensive, fast, quantitative and can be performed simply on relatively inexpensive equipment. Another technology, which hasn't been exploited to any great extent in pesticide urinary analyses is fluorescence microbead Immunoassay (FMIA). FMIAs can be multiplexed, using beads which can be identified by differing ratios of internal fluorescence. In a multiplexed assay, individual sets of microspheres are conjugated with the target molecules required for each reaction. A fluorescent antibody is prepared for each target molecule. The fluorescent antibodies are mixed to form a cocktail for the multiplexed reactions. The microspheres are then reacted with a mixture of analytes, such as a urine sample, followed by the cocktail of fluorescent antibodies. In the present work we describe 3 multiplexed FMIA assay for alar, alachlor, metalachlor mercapturate and atrazine.
Pesticides; Agricultural chemicals; Quantitative analysis; Chemical analysis; Urinalysis; Metabolites; Pesticides and agricultural chemicals
Abstract; Conference/Symposia Proceedings
Proceedings of the 2000 International Chemical Congress of Pacific Basin Societies, December 14-19, 2000, Honolulu, Hawaii