Antioxidant properties of (-)-epicatechin-3-gallate and its inhibition of Cr(VI)-induced DNA damage and Cr(IV)- or TPA-stimulated NF-kB activation.
Shi-X; Ye-J; Leonard-SS; Ding-M; Vallyathan-V; Castranova-V; Rojanasakul-Y; Dong-Z
Mol Cell Biochem 2000 Mar; 206(1-2):125-132
Electron spin resonance (ESR) spin trapping was utilized to investigate the scavenging effects on hydroxyl radicals (OH) and superoxide radicals (O2-) by (-)-epigallocatechin-3-gallate (EGCG), one of the major anticancer compounds in tea. The spin trap used was 5,5-dimethyl-pyrroline N-oxide (DMPO). The Fenton reaction (Fe2+ + H2O2-->Fe3+ + OH + OH-) was used as a source of OH radicals. EGCG efficiently scavenges OH radicals with reaction rate of 4.62 x 10(11) M(-1)sec(-1), which is an order of magnitude higher than several well recognized antioxidants, such as ascorbate, glutathione and cysteine. It also scavenges O2- radicals as demonstrated by using xanthine and xanthine oxidase system as a source of O2- radicals. Through its antioxidant properties, EGCG exhibited a protective effect against DNA damage induced by Cr(VI). EGCG also inhibited activation of nuclear transcription factor NF-kappaB induced by Cr(IV) and 12-o-tetradecanoylphorbol-13-acetate (TPA). The present studies provide a mechanistic basis for the reported anticarcinogenic properties of EGCG and related tea products.
Antioxidants; Antioxidation; Cancer; Free-radical-generation; Free-radicals; Hydroxyl-groups
Molecular and Cellular Biochemistry