Antioxidant properties of pyrrolidine dithiocarbamate and its protection against Cr(VI)-induced DNA strand breakage.
Shi-X; Leonard-SS; Wang-S; Ding-M
Ann Clin Lab Sci 2000 Apr; 30(2):209-216
Pyrrolidine dithiocarbamate (PDTC) is considered an antioxidant and is frequently used to study the role of free radical reacions in various biological processes and against free radical-induced cellular injuries. However, its antioxidant properties are not characterized. In this study, electron spin resonance (ESR) was used to investigate the antioxidant potential of PDTC with hydroxyl radical (OH) and superoxide anion radicals (O2-). The Fenton reaction [Fe(II) + H2O2 --> Fe(II) + OH + OH-)] and xanthine and xanthine oxidase were used as sources of OH and O2- radicals, respectively. The results show that PDTC effectively scavenges OH radicals with a reaction rate constant of approximately 2.73 x 10(10) M(-1) s(-1), which is comparable to other efficient OH radical scavengers, such as ascorbate and glutathione. PDTC is also able to scavenge O2- radicals. Through its antioxidant properties, PDTC protects against Cr(VI)-induced DNA strand breakage.
Antioxidants; Antioxidation; Free-radicals; Free-radical-generation; Cellular-reactions; Hydroxyl-groups;
Author Keywords: Pyrrolidine dithiocarbamate; antioxidant; hydroxyl radical; superoxide radical; Cr(VI); DNA damage
Xianglin Shi, Ph.D., Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Mail Stop B167, Morgantown, WV 26505, USA
Annals of Clinical and Laboratory Science