Methods of assessing the percutaneous absorption of volatile chemicals in isolated perfused skin: studies with chloropentafluorobenzene and dichlorobenzene.
Riviere-JE; Brooks-JD; Qiao-G
Toxicol Methods 2000 Oct; 10(4):265-281
The experimental determination of dermal absorption of volatile chemicals is fraught with difficulties. The isolated perfused porcine skin flap (IPPSF) is a biologically intact, perfused skin preparation that has been employed to predict dermal absorption of chemicals in humans. The purpose of this work was to explore various experimental dosing strategies for volatile chemicals using dichlorobenzene (DCB) and chloropentafluorobenzene (CPFB) as model compounds. Effects of complete occlusion and various strategies of vapor trapping, vapor dosing, and solvent effects were explored. The results suggest that dosing methodology is a major determinant of dermal absorption and could easily skew results obtained from different systems. A biologically sensitive system such as the IPPSF is particularly sensitive to the manipulations required to ensure precise dosing of these compounds. An interesting finding was that the effects of solvents on compound absorption that are routinely described in liquid dosing scenarios were also detected when both the compound and solvent were exposed during the vapor phase.
Humans; Chemical-analysis; Solvents; Solvent-vapors; Biological-effects; Biological-factors; Absorption-rates;
Author Keywords: Chloropentafluorobenzene; Dermal Absorption; Dichlorobenzene; Volatile Chemicals
Jim E. Riviere, Center for Cutaneous Toxicology and Residue Pharmacology, College of Veterinary Medicine, 4700 Hillsborough Street, North Carolina State University, Raleigh, NC 27606, USA