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Requirement for DARPP-32 in progesterone-facilitated sexual receptivity in female rats and mice.
Mani SK; Feinberg AA; O'Callaghan JP; Snyder GL; Allen PB; Dash PK; Moore AN; Mitchell AJ; Bibb J; Greengard P; O'Malley BW
Science 2000 Feb; 287(5455):1053-1056
DARPP-32, a dopamine- and adenosine 3',5'-monophosphate (cAMP)-regulated phosphoprotein (32 kilodaltons in size), is an obligate intermediate in progesterone (P)-facilitated sexual receptivity in female rats and mice. The facilitative effect of P on sexual receptivity in female rats was blocked by antisense oligonucleotides to DARPP-32. Homozygous mice carrying a null mutation for the DARPP-32 gene exhibited minimal levels of P-facilitated sexual receptivity when compared to their wild-type littermates. P significantly increased hypothalamic cAMP levels and cAMP-dependent protein kinase activity. These increases were not inhibited by a D1 subclass dopamine receptor antagonist. P also enhanced phosphorylation of DARPP-32 on threonine 34 in the hypothalamus of mice. DARPP-32 activation is thus an obligatory step in progestin receptor regulation of sexual receptivity in rats and mice.
Laboratory-animals; Animals; Animal-studies; Genetic-factors; Genes; Gene-mutation; Reproductive-effects; Reproductive-system-disorders
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