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Effects of various treatments on the quantitative recovery of endotoxin from water-soluble metalworking fluids.
Brown ME; White EM; Feng HA
Am Ind Hyg Assoc J 2000 Jul/Aug; 61(4):517-520
Three extraction methods were compared for their effectiveness in the quantitative removal of endotoxin from unused and used bulk water-soluble metalworking fluid (MWF) samples. Soluble, synthetic, and semisynthetic fluids were studied. The three modes of extraction consisted of (1) pyrogen-free water (PFW); (2) PFW and Tween 20 (polyoxyethylene sorbitan monolaurate); and (3) PFW, Tween 20, and sonication. Results suggest that vigorous recovery methods yield higher amounts of endotoxin from MWF samples than mild recovery methods in PFW alone. Additional studies are required to aid in the understanding of the factors that significantly affect endotoxin extraction yields from these fluids.
Endotoxins; Metalworking fluids; Bioassays; Analytical methods; Analytical processes; Microorganisms; Author Keywords: endotoxin; Limulus amoebocyte lysate (LAL) assay; metalworking fluids
U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Division of Physical Sciences and Engineering, 4676 Columbia Parkway, Cincinnati, OH 4
Issue of Publication
American Industrial Hygiene Association Journal
Page last reviewed: June 15, 2021
Content source: National Institute for Occupational Safety and Health Education and Information Division