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Nitric oxide inhalation transiently elevates pulmonary levels of cGMP, iNOS mRNS, and TNF-alpha.
Brady TC; Crapo J; Mercer RR
Am J Physiol, Lung Cell Mol Physiol 1998 Sep; 275(3):L509-L515
The initial pulmonary vasodilation that occurs during nitric oxide. (NO) inhalation does not appear to be maintained chronically in many cases. NO may acutely relax vascular smooth muscle by increasing levels of guanosine 3',5'-cyclic monophosphate (cGMP), tumor necrosis factor (TNF) -alpha, and inducible nitric oxide synthase (iNOS) while decreasing levels of lipid peroxidation. It was hypothesized that the acute NO-induced changes in cGMP, TNF-alpha, iNOS, and lipid peroxidation, all of which may mediate vasodilation, are transient rather than sustained. Lungs from rats kept in chambers containing 6 parts/million. NO for 1 h, 1 day, or 1 wk were analyzed for levels of NO-induced vasodilatory mediators. Pulmonary cGMP, iNOS mRNA, and TNF-alpha were increased 1 h after NO exposure but decreased to control values at later times. Levels of malonyl dialdehyde, an indicator of lipid peroxidation, were decreased at all times during NO inhalation. As a whole, the data suggest that in lungs the vasodilatory mediators cGMP, iNOS, and TNF-alpha are only acutely and transiently elevated during inhalation of NO, consistent with the initially positive clinical response to inhaled NO that deteriorates over time.
Gas-inhalation; Chronic-exposure; Muscle-relaxation; Rats; Biological-models; Author Keywords: lung; pulmonary hypertension; critical care medicine; interferon-g; superoxide dismutase
Todd C. Brady, Program in Integrated Toxicology, Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710
10102-43-9; 542-78-9; 7665-99-8; 82115-62-6
Issue of Publication
American Journal of Physiology: Lung Cellular and Molecular Physiology
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