Neonatal exposure to technical methoxychlor alters pregnancy outcome in female mice.
Reprod Toxicol 1998 Nov; 12(6):565-573
This study was designed to determine the ability of female mice who were exposed neonatally to the pesticide methoxychlor (MXC) to mate, ovulate, and become pregnant upon reaching sexual maturity. One-day-old female mice (5to 8/group) were exposed daily by intraperitoneal (ip) injection for 14d to either sesame oil or 10 microgram estrodiol-17B or 0.1, 0.5 or 1.0 mg MXC suspended in sesame oil. The MXC exposures corresponded to 14 to 71, 68 to 357, or 145 to 714 mg/kg body weight, respectively. Three months later, female mice were placed with proven breeder males and checked daily for vaginal plugs. Mated female mice were sacrificed 18d after the appearance of a vaginal plug to evaluate pregnancy. Uteri were examined for the presence of living fetuses and/or resorption sites. Ovaries were removed and prepared for histologic evaluation and tabulation of corpora lutea. All mice from all three MXC-treated groups did in fact mate, in comparison with only one of those exposed neonatally to estradiol. Increasing the dose of MXC produced a decreased number of pregnant animals at 18d following mating. The mean number of live fetuses/litter was reduced in the 0.5 and 1.0 mg MXC-treated groups. Corpora Lutea were significantly reduced in ovaries from only the 1.0 mg MXC group and the estradiol. No effects of treatment were seen at 1.0 mg MXC. It is concluded that neonatal exposure to MXC does not interfere with mating. Instead, significant alterations are seen in initiating and/or maintaining pregnancy. The deleterious effects on pregnancy may be due to the influence of neonatal MXC treatments on the hypothalamic-pituitary-ovarian axis as well as on possible alteration of the uterine environment.
Chlorinated-hydrocarbons; Toxicology; Uterus; Reproductive-system-disorders; Fertility
Department of Cell Biology and Anatomy, LSU Medical Center, 1901 Perdido Street, New Orleans, LA 70112-1393
Louisiana State University, School of Medicine, Department of Anatomy