Mineral Surface-specific Differences in the Adsorption and Enzymatic Removal of Surfactant and Their Correlation With Cytotoxicity.
Wallace-WE Jr.; Keane-MJ; Mike-PS; Et Al
Springer-Verlag 1989 :50-56
Respirable quartz dust and a kaolin clay dust have been found to be of comparable cytotoxic potential in vitro on a specific surface area basis, despite the distinctly different potentials of quartz and clay for causing pneumoconiosis or pulmonary fibrosis. A respired particle depositing in a respiratory bronchiole or alveolus will contact a pulmonary surfactant hypophase, of which a primary component is diacyl glycerophosphorylcholine, or diacyl lecithin. Several studies have found that adsorption of such surfactant suppresses in vitro cytotoxic effects of quartz dust. Subsequent phagocytosis and digestion by a macrophage may remove the coating, restoring cytotoxic potential and initiating disease processes. Adsorption of dipalmitoyl lecithin from emulsion in physiologic saline by respirable sized quartz dust and kaolin dusts, and consequent diminution of the dusts' hemolytic and macrophage cytotoxic potential, have been reported. This report presents additional data on the cell-free in vitro phospholipase a2 enzymatic digestion of dipalmitoyl lecithin adsorbed on a quartz and a kaolin respirable-sized dust to investigate mineral-specific differences in the rates of digestion and of consequent restoration of dust membranolytic potential.
OP; Final Contract Report;
Springer-Verlag, 1989, PP. 50-56
West Virginia University