Nursery Techniques for Production of Tree Seedlings Infected With Specific Mycorrhizal Fungi for Surface Mine Reclamation.
For Reference Only At Bureau Libraries :45 pages
A survey of endomycorrhizal fungi occurring on orphan coal mine lands in western and eastern Kentucky revealed a great diversity of species present. Thus, a large number of fungi are available for isolation and evaluation for enhancing revegetation of mined lands. Endomycorrhizal fungi isolated from orphan mines stimulated growth of sweetgum seedlings on mine soil at low fertilization but inhibited at high fertilization; this growth inhibition at high fertilization did not occur with plants growing on peat-perlite. Banding of endomycorrhizal inoculum was not advantageous, compared with incorporation of inoculum throughout the growth medium, for production of mycorrhizal sweetgum seedlings. Newly reclaimed mine spoils were nearly nonmycorrhizal, compared with land reclaimed 4 or 8 years earlier. A procedure for isolation of single-spore cultures of endomycorrhizal fungi was developed; this is necessary for ecotypic variability. A practical procedure using container-grown pisolithus tinctorius-infected shortleaf pine seedlings for production of large numbers of bareroot pine seedlings infected with p.Tinctorius for mined land reclamation was demonstrated.
CP; Final Contract Report;
For Reference Only At Bureau Libraries
Univ. of Kentucky