Protein damage caused by occupational toxicants.
Division of Toxicology, University of Arkansas for Medical Sciences, University of Arkansas, Little Rock, Arkansas 1997 Jun; :1-9
Studies were conducted to determine whether certain occupational toxicants are capable of causing tissue damage by oxidatively modifying proteins and that these oxidized proteins can be detected using polyclonal antibody techniques to provide a measure of exposure assessment. To investigate this theory, an immunochemical assay was developed to detect oxidative protein damage using a polyclonal antibody assay and validated by direct comparison with the established spectrophotometric techniques. The utility of the immunoassay was then tested to detect oxidized proteins in animals exposed to oxidants found in the occupational environments. The findings indicated that the technique is valid for immunochemical determination of protein oxidation. The technique could be used to determine carbonyl groups formed by oxidation of protein by either metal catalyzed or radiolytically generated oxygen radicals. A comparison of the technique with the spectrophotometric assay indicates that oxidative damage can be detected immunochemically using three orders of magnitude less protein. The results further suggest that the technique has limited usefulness for identifying oxidative damage to protein tissue fractions from tissue treated in- vitro or in-vivo, primarily due to the high background of oxidized proteins that occur during normal cellular physiology.
NIOSH-Grant; Pulmonary-system-disorders; Oxidative-processes; Respiratory-system-disorders; Lung-irritants; Immunology; Immunological-tests; Biological-monitoring; Antibody-response; Occupational-exposure
Pharmacology-Toxicology Univ of Arkansas for Medical S 4301 West Markham, Slot 638 Little Rock, AR 72205
Final Grant Report
NTIS Accession No.
Pulmonary System Disorders
Division of Toxicology, University of Arkansas for Medical Sciences, University of Arkansas, Little Rock, Arkansas
University of Arkansas Med Scis Ltl Rock, Little Rock, Arkansas