Investigation of ornithine decarboxylase activity and two-dimensional electrophoretic protein profile following exposure of T24 bladder carcinoma cells to tumor promoter and carcinogen.
Kanitz-MH; Li-EI; Schulte-PA; Anderson-NL; Rothman-N; Savage-RE Jr.
Toxicol Methods 1997 Jan; 7(1):27-41
Changes were investigated in T24 human bladder carcinoma cell ornithine-decarboxylase activity and protein profiles in an effort to develop appropriate screening tools for biomarkers of effects of exposure to occupational chemicals. A unique chromatographic approach was used to demonstrate that in-vitro exposure of T24 cells for 6 hours to varying concentrations of 4-aminobiphenyl (92671) elevated enzyme activity 5.3 to 5.9 fold. Two dimensional gel electrophoresis was also used to compare the protein pattern of vehicle control treated T24 to 4-aminobiphenyl or 12-o- tetradecanoylphorbol-13-acetate treated cells. The Kepler software package was used to determine changes in abundance and modification of proteins. Protein markers were identified by significant changes in spot density following T24 treatment with either chemical. Fifteen protein spots from a detectable pool of 542 demonstrated two fold or greater changes in intensity. These findings illustrate the potential of automated two dimensional gel analysis for classifying different gel patterns. In addition to the ornithine-decarboxylase assay, two dimensional polyacrylamide gel electrophoresis offers promise for evaluating potential biomarkers for occupational and environmental carcinogens. The authors suggest that their findings will assist NIOSH in their efforts in the molecular epidemiology of occupational bladder carcinogenesis.
Bladder-cancer; Epidemiology; Protein-chemistry; Biological-monitoring; Cancer-rates; Risk-factors; Analytical-methods; Chemical-analysis; Analytical-chemistry
Infectious Diseases; Disease and Injury