Pharmacologic studies of paper dust extracts in isolated guinea pig trachea.
Authors
Schachter-E; Zuskin-E; Buck-MG; Maayani-S; Goswami-SK; Rienzi-N; Ali-S; Wan-E; Castranova-V; Siegel-P; Whitmer-M
Source
Cotton Dust: Proceedings of the Twentieth Cotton and Other Organic Dusts Research Conference, 1996 Beltwide Cotton Conferences. Wakelyn P, Jacobs R, Rylander R, eds., Memphis, TN: National Cotton Council, 1996 Jan; :363-365
Abstract
The pharmacologic properties of water soluble extracts of paper dust (PDE) were investigated using isolated guinea-pig trachea. Dose response relationships between PDEs and contraction of guinea-pig tracheal smooth muscle were established. Paper dust was obtained from separate sites in a paper recycling mill, both early and later in the process. Pretreatment with mediator blockers, arachidonic- acid metabolite inhibitors, and an intracellular calcium antagonist were examined and compared to findings with similar experiments using cotton bract extracts. The effect of pretreatment with capsaicin was also assessed. Contraction induced by paper dust extract collected early in the process was partially inhibited by all the mediator inhibiting agents as well as the receptor blocking agents with the exception of LY-171883. Similar findings were noted for paper dust extract collected late in the process, except that pyrilamine and indomethacin failed to block the bronchoconstrictor effect. Pretreatment with capsaicin enhanced the effects of both early and late paper dust extract. The authors suggest that water soluble extracts from the paper recycling industry cause airway smooth muscle constriction via mediator release.
Keywords
NIOSH-Publication; NIOSH-Grant; Laboratory-animals; Respiratory-system-disorders; Lung-irritants; In-vitro-studies; Alveolar-cells; Lung-cells; Cytotoxic-effects; Organic-dusts
Contact
Medicine Mount Sinai Medical Center One Gustave L Levy Place New York, N Y 10029
Document Type
Conference/Symposia Proceedings
Editors
Wakelyn-P; Jacobs-R; Rylander-R
Identifying No.
Grant-Number-R01-OH-02593
Priority Area
Pulmonary-system-disorders
Source Name
Cotton Dust: Proceedings of the Twentieth Cotton and Other Organic Dusts Research Conference, 1996 Beltwide Cotton Conferences
Performing Organization
Mount Sinai School of Medicine, New York, New York
