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Response of an animal model to mixtures of endotoxin and N-formyl-methionyl-leucyl-phenylalanine (FMLP) aerosols.
Frazer-DG; Robinson-VA; Siegel-PD; Al-Humadi-N; Afshari-AA; Goldsmith-WT; Olenchock-S; Whitmer-MP; Castranova-V
Cotton Dust: Proceedings of the Twentieth Cotton and Other Organic Dusts Research Conference, 1996 Beltwide Cotton Conferences. Wakelyn P, Jacobs R, Rylander R, eds., Memphis, TN: National Cotton Council, 1996 Jan; :360-363
The extent to which n-formyl-methionyl-leucyl-phenylalanine (FMLP) modifies the guinea-pig response to endotoxin was studied, and an attempt was made to determine if activating or deactivating the cellular response associated with endotoxin exposure is a function of FMLP. The breathing rates of English-short-hair-guinea-pigs inspiring 10% carbon-dioxide in air, after a 3 minute equilibrium period, were determined for four groups of animals: exposed to filtered air, FMLP, endotoxin, or a combination of FMLP and endotoxin. Breathing rates were determined preexposure, immediately post exposure, and 18 hours following exposure. The results indicated that the breathing rate of guinea-pigs exposed to FMLP increased immediately after exposure and then gradually decreased toward control values at 18 hours after exposure. The breathing rate of guinea-pigs exposed to endotoxin alone peaked later than the breathing rate of guinea-pigs exposed to FMLP alone. Animals exposed to both FMLP and endotoxin had a breathing rate response that was more like those animals exposed to FMLP alone than to animals exposed to endotoxin alone. The total number of cells recovered from lungs by lavage was greatest in animals exposed to endotoxin alone, the next greatest in animals exposed simultaneously to FMLP and endotoxin, then to FMLP alone, and finally controls. Macrophages from animals exposed simultaneously to FMLP and endotoxin were activated the most in terms of reactive species production. Since FMLP and endotoxin are both found in cotton dust, interactions between these two agents are thought to be important in the overall animal model response to cotton dust.
Cotton-dust; Alveolar-cells; Laboratory-animals; Organic-dusts; Breathing; Respiratory-system-disorders; Lung-irritants; Lung-cells; Plant-dusts; Cytotoxic-effects
Wakelyn-P; Jacobs-R; Rylander-R
Cotton Dust: Proceedings of the Twentieth Cotton and Other Organic Dusts Research Conference, 1996 Beltwide Cotton Conferences
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Content source: National Institute for Occupational Safety and Health Education and Information Division