Immunohistochemical localization of transforming growth factor beta isoforms in asbestos-related diseases.
Jagirdar-J; Lee-TC; Reibman-J; Gold-LI; Aston-C; Begin-R; Rom-WN
Environ Health Perspect 1997 Sep; 105(Suppl 5):1197-1203
The possibility that transforming growth factor beta (TGF-beta) would promote matrix accumulation in asbestosis, pleural plaques, and mesothelioma, and that the TGF-beta isoforms may have differential roles in this process were investigated. Autopsy lung tissue was taken from 16 individuals exposed to chrysotile (12001295) asbestos in the mines and mills of Quebec, Canada. All of the workers had significant chrysotile exposure, and had histologic evidence of pulmonary fibrosis. Five of the subjects had lung carcinoma and four had malignant mesothelioma. The examination showed increased immunolocalization of all three TGF-beta isoforms in the fibrotic lesions of asbestosis and pleural fibrosis. The hyperplastic type-II pneumocytes contained all three isoforms. As the fibrosis progressed, TGF-beta isoform immunoreactivity markedly increased parallel to the increase of extracellular matrix, reactive hyperplastic type-II pneumocytes, and macrophages. By contrast, there was differential spatial immunostaining for the TGF-beta isoforms in malignant mesothelioma, with TGF-beta1 in the stroma but TGF-beta2 in the tumor cells. The data found were consistent with an important role for TGF-beta in accumulation of extracellular matrix and cell proliferation in asbestos related diseases. The authors suggest that TGF-beta isoforms are important for the accumulation of extracellular matrix in asbestosis, pleural fibrosis, and malignant mesothelioma.
NIOSH-Publication; NIOSH-Cooperative-Agreement; Pulmonary-system-disorders; Humans; Occupational-respiratory-disease; Asbestos-fibers; Body-burden; Tissue-distribution; Lung-cancer
Asthma and Chronic Obstructive Pulmonary Disease; Disease and Injury; Pulmonary-system-disorders
Environmental Health Perspectives