The use of a second generation video imaging technique for assessing dermal exposure (VITAE) system was examined. The application of the VITAE system involved the following operational procedures: system stabilization, data acquisition, preexposure screening, postexposure screening, qualitative exposure evaluation, image preparation, image outline and overlay, image classification, image comparison, exposure calculation, image analysis, and final reporting. In terms of equipment, the VITAE system consisted of ultraviolet lamps and a television camera linked to a microcomputer with digital imaging capabilities and custom designed software. The florescent whitening agent 2,2'-(2,5-thiophenediyl)-bis(5-tert-butylbenzoxazole) (7128645) was the preferred fluorescent tracer. During field studies conducted in central Florida, the stability of the VITAE system during data acquisition was determined by periodically obtaining images of a standard fluorescent target. The precision of subject positioning and image outlining was determined in the laboratory. The sensitivity, specificity, and predictive value of computer based image classification criteria were determined by comparing computer generated results with visual observations. The effect of skin pigmentation on fluorescence was studied with subjects of various pigmentation. Data indicated that the VITAE system was highly stable throughout the data acquisition period and over the course of the 22 day field study. In general, image outlining and subject positioning variability contributed no more than 5% to the variability in exposure quantification. For the computer classification criteria, the diffuse exposure criterion had greater sensitivity, whereas the intense exposure criterion had greater specificity. The predictive value of the intense exposure criterion was higher than that of the diffuse exposure criterion. The effect of skin pigmentation on changes in the fluorescence response could be represented mathematically. The authors conclude that the second generation VITAE system may be used for the reliable, accurate and precise quantification of occupational dermal exposure.