Application of the gas chromatography-fatty scid methyl ester system for the identification of environmental and clinical isolates of the family Micrococcaceae.
Appl Occup Environ Hyg 1997 Aug; 12(8):543-546
The gas chromatography (GC) fatty acid methyl ester (FAME) microbial identification system (MIS) for characterizing environmental and clinical staphylococci and related organisms was evaluated. Bacteria from the American Type Culture Collection were used in this study. About 40mg of the bacterial colonies underwent saponification to convert their cellular fatty acids to fatty acid methyl esters. Of the 98 cultures obtained for the study, 17% were originally clinical isolates and 83% were environmental isolates. Differences in the FAME profiles of the Staphylococcus and Micrococcus species were sufficient to distinguish between closely related species. Bacteria were cultured, and FAME analytes were prepared. GC analysis of FAME analytes was conducted, using a flame ionization detector. The identification of bacteria by SIs using the MIS was based on the average FAME profiles of about 100 geographically different isolates for each test strain analyzed. Analysis of four old cultures showed that similarity indices (SI) fell as cultures aged. Based on historical analysis, an SI of 0.3 or greater was acceptable. Of the 98 strains, 96 were correctly identified, and another was identified, but with an SI below the 0.3 threshold. The authors conclude that the GC/FAME method of bacterial identification could serve as a primary means for identifying culturable Staphylococcus and Micrococcus species from bulk and air samples collected during indoor environmental quality investigations.
NIOSH-Author; Microbiology; Microorganisms; In-vitro-studies; Cell-cultures; Indoor-air-pollution; Chromatographic-analysis; Indoor-environmental-quality
Applied Occupational and Environmental Hygiene