Selective hyperexpression of c-jun oncoprotein by glass fiber- and silica-transformed BALB/c-3T3 cells.
Gao-H; Brick-J; Ong-S; Miller-M; Whong-Z; Ong-T
Cancer Lett 1997 Jan; 112(1):65-69
A study was conducted exploring the relationship between silica (7631869) and glass fiber induced cell transformation and oncoprotein expression. The production of the protein products of seven protooncogenes and one tumor suppressor gene was studied by immunoblotting in BALB/c-3T3 cells transformed by exposure to glass fibers or silica. The level of the protooncogene c-jun protein was increased in all glass fiber and silica transformed cells but not in two spontaneously transformed cell lines. No protein products of the protooncogenes c-K-ras, c-myc, or c-sis were detectable in transformed or nontransformed cells; levels of the protein products of the c-H-ras, p53, c-erb B1, and c-myb genes were similar between transformed and nontransformed cells. The authors conclude that overexpression of c-jun may be associated with glass fiber and silica induced transformation of BALB/c-3T3 cells.
NIOSH-Author; Silica-dusts; Fibrous-glass; Cell-transformation; Carcinogenicity; Protein-synthesis; Mineral-dusts;
Author Keywords: Mineral particles; Cell transformation; BALB/c-3T3; Oncoproteins; Western blotting