Asbestos induced alteration in endothelial cell function.
The Environmental Affairs Group, Sanders A. Lockheed Martin Company, Nashua, New Hampshire 1997 Jan; :1-6
The purpose of this research was to investigate whether asbestos (1332214) induces an active endothelial cell phenotype, resulting in the increased expression of growth factors and proteases, which are relevant to the development of fibrosis. Porcine aortic endothelial cells were cultured with asbestos and ceramic fibers. The findings indicated that endothelial cells respond to noncytotoxic concentrations of asbestos fibers with alterations in specific cell functions, which may be relevant to pulmonary disease processes. The cells respond to both chrysotile (12001295) and crocidolite (12001284) asbestos fibers by changing cell morphology in focal areas of fiber contact, assuming a spindled appearance, indicative of the activated endothelial cell. A time dependent increase was noted in neutrophil binding to Intercellular-Adhesion-Molecule-1 and increases in both Urokinase-like-Plasminogen-Activator (uPA) mRNA levels and uPA activity. Endothelial cells exposed to chrysotile asbestos demonstrated in a time dependent manner an increase in the expression of the cell surface uPA receptor. Exposed cells also demonstrated an increase in the activation of the transcription activating factor Nuclear-Factor-kappa-B (NF-kB). Refractory-Ceramic- Fiber-Type-1 did not stimulate the endothelial cell morphology alteration, increase PMN binding, uPA mRNA or activity, or increase NF-kB nuclear translocation.
NIOSH-Grant; Pulmonary-system-disorders; Asbestos-fibers; Fibrous-dusts; Lung-irritants; Respiratory-system-disorders; Cytotoxic-effects; Cell-cultures; Mammalian-cells
Pharmacology and Toxicology Dartmouth Medical School Remsen 7650 Hanover, NH 03755-3835
1332-21-4; 12001-29-5; 12001-28-4
Final Grant Report
NTIS Accession No.
The Environmental Affairs Group, Sanders A. Lockheed Martin Company, Nashua, New Hampshire
Dartmouth College, Hanover, New Hampshire