Effects of ozone upon macrophage-interferon interactions.
Cohen MD; Zelikoff JT; Qu Q; Schlesinger RB
Toxicology 1996 Dec; 114(3):243-252
The effects of ozone (10028156) upon macrophage/interferon interactions were examined. Cultures of WEHI-3 cells, a Balb/c- mouse macrophage like cell line, were exposed to 1 part per million ozone for 4 hours. They were then treated with 0.005 to 1 nanomolar phosphorus-32 labeled or unlabeled interferon-gamma (INFg). The extent of cell/INFg binding was determined. Other exposed cells after priming with serum opsonized zymosan (SOZ) were analyzed for their ability to produce superoxide anion and hydrogen-peroxide. The effects on intracellular calcium ion (Ca2+) homeostasis and INFg class II/I-A antigen expression were determined. The ability of some cells to phagocytize opsonized polystyrene latex microspheres was evaluated. Ozone exposure increased the level of total cell/INFg binding by 62 to 67%. Ozone significantly decreased SOZ stimulated production of superoxide and hydrogen-peroxide. Ozone decreased the phagocytic activity of cells cultured in the presence of INFg. Ozone significantly decreased the increases in Ca2+ flux across the cell membrane induced by INFg. Ozone did not significantly affect INFg enhanced expression of class II/I-A antigens. The authors conclude that in-vitro ozone exposure can alter the capacity of WEHI-3 cells to bind INFg; however, its effect upon INFg inducible responses are variable. This suggests that in- vivo ozone exposure would probably alter the ability of macrophage like cells to interact with, and be activated by INFg, which would consequently impact upon other macrophage dependent system cells during times of host antigen or bacterial challenge.
Toxic-gases; Mammalian-cells; Phagocytic-activity; Physiological-response; In-vitro-studies; Immune-system; Chemical-binding
Environmental Medicine New York University Med Center 550 First Ave New York, NY 10016
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New York University, New York, New York