Nitric oxide alters metabolism in isolated alveolar type II cells.
Miles-PR; Bowman-L; Huffman-L
Am J Physiol, Lung Cell Mol Physiol 1996 Jul; 271(1):L23-L30
The effects of the nitric-oxide (10102439) generator S-nitroso-N- acetyl-penicillamine (SNAP) and the nitric-oxide-synthase (NOS) inhibitor NG-nitro-L-arginine-methyl-ester (L-NAME) on the synthesis of lung surfactant dipalmitoyl-phosphatidylcholine (DPPC), and some basic processes involved in energy metabolism were investigated. The study also examined the effects of some NOS inhibitors and external calcium on cellular nitric-oxide free radical production. Studies were conducted using alveolar type-II cells isolated from male Sprague-Dawley-rats. The findings demonstrate that the nitric- oxide (10102439) levels in alveolar type-II cells are able to affect lipid and energy metabolism. Exposure of the cells to nitric-oxide radicals from an external source causes reductions in disaturated phosphatidylcholines (DSPC) synthesis, cell ATP levels, and cellular oxygen consumption. However, inhibition of endogenous nitric-oxide radical levels induced by L-NAME, resulted in increased DSPC synthesis, cell ATP levels, and cellular oxygen consumption. It was also suggested that at least some of the endogenous nitric-oxide radicals may be generated by cNOS activity but that the precise regulatory role of NOS was not yet clear.
NIOSH-Author; Metabolic-study; Lung-irritants; Enzyme-activity; Surfactants; Free-radicals; Respiratory-system-disorders; Alveolar-cells; Mammalian-cells; In-vitro-studies;
Author Keywords: lung surfactant; adenosine 5' triphosphate
P. R. Miles, Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown 26505
American Journal of Physiology: Lung Cellular and Molecular Physiology