Quantification of epithelial cell micronuclei by fluorescence in situ hybridization (FISH) in mortuary science students exposed to formaldehyde.
Titenko-Holland N; Levine AJ; Smith MT; Quintana PJ; Boeniger M; Hayes R; Suruda A; Schulte P
Mutat Res 1996 Dec; 371(3-4):237-248
A study was conducted examining the incidence of micronucleated cells among mortuary science students intermittently exposed to formaldehyde (50000). Cytopathological studies were conducted on buccal and nasal cells obtained from 28 students before and after known formaldehyde exposure in an embalming class. At least one micronucleated cell was identified in preexposure buccal cell samples in 60% of the subjects tested, whereas 90% of the subjects had at least one micronucleated cell in postexposure samples. In addition, the number of cells with more than one micronucleus was increased following formaldehyde exposure. The mean frequency of centromere negative micronucleated cells was increased nine fold and the mean frequency of centromere positive micronucleated cells was increased more than two fold following exposure to embalming fluid. A weak association was identified between cumulative exposure to embalming fluid and the change in total micronucleus frequency. Formaldehyde exposure did not significantly affect the total micronucleus frequency in nasal epithelial cells; however, as seen in buccal cells, the increase in micronucleus frequency was greater for centromere negative micronuclei than for centromere positive micronuclei. The authors conclude that the finding that chromosome breakage appears to be the primary mechanism of micronucleus formation is consistent with the known clastogenic properties of formaldehyde.
Preservatives; Genotoxic-effects; Aldehydes; DNA-damage; Cell-alteration; Formaldehydes; Cytopathology; Exposure-levels; Exposure-assessment; Sampling;
Author Keywords: Exfoliated cell; Mortuary science student; Micronuclei; FISH; Formaldehyde
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