Detection of DNA adducts by electron capture mass spectrometry.
Chem Res Toxicol 1997 Mar; 10(3):255-270
Electron capture mass spectrometry (EC/MS) as a means of detecting DNA adducts was reviewed. The general concept of DNA adduction and its physiological significance were summarized. The general history of the development of EC/MS for detecting DNA adducts was outlined. Electrophores and their role in DNA adduct detection were discussed. The discussion considered the general concept of electrophores, conversion of DNA adducts into electrophores, electrophore subtypes, electrophore labels, the role of residual acidic hydrogen atoms in EC/MS analysis of DNA adducts, the types of derivatization reactions that can be used to convert DNA adducts into electrophores, the polar footprint concept as applied to EC/MS analysis of DNA adducts, and appropriate derivatization conditions for converting DNA adducts into electrophores. Specific applications of electrophore labeling for DNA adduct analysis by EC/MS were described. These include procedures for converting DNA adducts formed by 4-aminobiphenyl (92671), 4-aminofluorene (7083638), benzo(a)pyrene (50328), 4- (hydroxypyridyl)butanone, malondialdehyde (542789), 2- phosphoglycolate, and uracil (66228) into electrophores. Electrophores formed from compounds such as cytosine, methylcytosine, and hydroxymethyluracil were also described. The future prospects for EC/MS as a technique for determining DNA adducts were discussed.
NIOSH-Publication; NIOSH-Contract; NIOSH-Grant; Grants-other; DNA-adducts; Analytical-methods; Mass-spectrometry; Sample-preparation
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Chemical Research in Toxicology
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