NIOSHTIC-2 Publications Search

Development and evaluation of an inhalable bioaerosol manifold sampler.

Gao P; Dillon HK; Farthing WE
Am Ind Hyg Assoc J 1997 Mar; 58(3):196-206
An inhalable bioaerosol manifold sampler designed to collect replicate samples over sequential sampling periods was developed and evaluated. The sampler design consisted of 12 small stainless steel sample holders arranged in pairs around a conical inlet and flange. The inlet had a circumference of 73 cm. The flange served to straighten the streamlines in the inlet, minimizing impaction losses. Air flow through the filters when mounted in the sample holders was controlled by regulating a vacuum applied to a plenum. The 12 sample holders enabled replicate samples to be collected over six sequential sampling periods. The manifold sampler was tested in a wind tunnel using aerosolized monodisperse or polydisperse polystyrene beads having diameters of 2.0 to 25.6 micrometers (micron) at wind velocities 0.2 to 1.4 meters per second (m/sec). The data obtained with the sampler were compared with data obtained with an APS model 3310 aerodynamic particle sizer (APS). The manifold sampler was used to measure fungal spore concentrations in an experimental cotton carding room and in a shed at a dairy farm. The sampling efficiencies of the manifold sampler came within 10% of the American Conference of Governmental Industrial Hygienists (ACGIH) standard inhalable curve when collecting particles having diameters of up to 18micron at wind velocities below 0.6m/sec and a sampling velocity of 0.6m/sec in the wind tunnel. For particles larger than 18micron, the sampling efficiencies came within 18% of the ACGIH curve. At velocities greater than 0.6m/sec, sampling efficiencies came within 15 and 20% of the ACGIH curve for particle diameters of 22micron or less and larger than 22micron, respectively. Particle counts measured in polydisperse aerosols by the manifold sampler came within 92 to 115% of those measured by the APS. The manifold sampler performed satisfactorily in the field tests. The authors conclude that the manifold sampler is particularly suited for filter sampling of environments that are contaminated with biological dusts.
NIOSH-Publication; NIOSH-Author; NIOSH-Grant; Grants-other; Industrial-hygiene; Aerosol-sampling; Fungi; Air-sampling-equipment; Equipment-reliability; Laboratory-testing; Airborne-particles; Author Keywords: aerosol sampler; bioaerosol; inhalable size fraction; microscopy; sampling
Environmental Health Sciences University of Alabama Uab Station Birmingham, AL 35294
Publication Date
Document Type
Journal Article
Funding Amount
Funding Type
Fiscal Year
Identifying No.
Issue of Publication
NIOSH Division
Priority Area
Other Occupational Concerns; Grants-other
Source Name
American Industrial Hygiene Association Journal
Performing Organization
University of Alabama at Birmingham, Birmingham, Alabama
Page last reviewed: March 4, 2022
Content source: National Institute for Occupational Safety and Health Education and Information Division