In vitro effects of large and small glass fibers on rat alveolar macrophages.
Castranova-V; Pailes-W; Judy-D; Blake-T; Schwegler-Berry-D; Jones-W
J Toxicol Environ Health, A 1996 Nov; 49(4):357-369
The use of alveolar macrophage cultures to assess the cytotoxicity and fibrogenicity of two glass fibers, building insulation fiberglass (long and thick) and glass microfiber (short and thin) was examined. Alveolar macrophages were obtained from bronchoalveolar lavage fluid of male Sprague-Dawley-rats, and sterilized suspended fibers were added for 1, 2, or 3 days. The glass microfibers caused a concentration dependent decrease in cell viability as measured by trypan-blue exclusion. The building insulation fiberglass caused no significant loss of cell viability. The glass microfibers significantly increased lactate-dehydrogenase (LDH) and beta-galactosidase (BG) release in a concentration dependent manner, whereas the insulation fiberglass did not increase LDH or BG. The glass microfibers decreased oxygen consumption and hydrogen-peroxide release, whereas insulation fiberglass had no effect. The authors conclude that short and thin glass fibers are more toxic than long and thick fibers in-vitro, implicating fiber dimension as an important determinant of toxicity.
NIOSH-Author; Alveolar-cells; In-vitro-study; Laboratory-animals; Inhalation-studies; Asbestosis; Asbestos-fibers; Cytotoxic-effects; Lung-disorders; Lung-fibrosis; Cell-cultures;
Vincent Castranova, PhD, NIOSH-HELD, 1095 Willowdale Road, Morgantown, WV 26505-2888, USA
Journal of Toxicology and Environmental Health, Part A: Current Issues