Vanadium affects macrophage interferon-gamma-binding and -inducible responses.
Cohen-MD; McManus-TP; Yang-Z; Qu-Q; Schlesinger-RB; Zelikoff-JT
Toxicol Appl Pharmacol 1996 May; 138(1):110-120
The effects of vanadium (7440622) on macrophage interferon-gamma (INFg) binding and inducible responses were examined. Mouse WEHI-3 cells, a macrophage like cell line, primed with 50 to 1,000 picomolar (pM) iodine-125 tagged INFg or unprimed were incubated overnight with 0 or 100 nanomolar (nM) or 100 micromolar (microM) ammonium-metavanadate (7803556) (AMV) or vanadium-pentoxide (1314621) at 22 degrees-C. The effects on INFg binding to cellular receptors were determined. Production of superoxide-anion (O2-) and hydrogen-peroxide in response to the treatments was measured before and after the cells were stimulated with zymosan. AMV decreased the level of intracellular INFg binding by 20 to 30% at the INFg concentrations used; however, none of the decreases were statistically significant. Scatchard plots of the AMV binding data indicated that the cells had two classes of binding sites, with an inflection point occurring at 100pM INFg. AMV decreased the number of class-I INFg receptors by 48%, but increased the affinity of the remaining receptors by 1.45 times the control value. AMV did not affect the number of class-II receptors, but increased their binding affinity 2 fold. Basal and zymosan stimulated production of O2- and hydrogen-peroxide were significantly increased by both AMV and vanadium-pentoxide. The relative increases in zymosan stimulated O2- and hydrogen-peroxide production were lower than in control cells. Prior to priming with INFg, calcium ion (Ca2+) concentrations in cells treated with 100microM AMV or vanadium-pentoxide were significantly greater than in control cells. Addition of INFg significantly decreased Ca2+ concentrations. In cells treated with either compound at 100microM, the number of cells expressing I-A surface antigen was significantly increased following INFg treatment. No changes in I-A antigen expression were seen when INFg was added to cells treated with 100nM AMV or vanadium-pentoxide. The authors conclude that in-vitro exposure to vanadium can alter the capacity of WEHI-3 cells to bind and respond to IFNg.
NIOSH-Publication; NIOSH-Grant; Grants-other; Alveolar-cells; In-vitro-studies; Transition-metals; Vanadium-compounds; Immune-system; Mammalian-cells; Chemoreceptors; Chemical-binding
Environmental Medicine New York University Med Center 550 First Ave New York, NY 10016
7440-62-2; 7803-55-6; 1314-62-1
Other Occupational Concerns; Grants-other
Toxicology and Applied Pharmacology
New York University, New York, New York