Increased neutrophil adherence to endothelial cells exposed to asbestos.
Treadwell-MD; Mossman-BT; Barchowsky-A
Toxicol Appl Pharmacol 1996 Jul; 139(1):62-70
Porcine aortic endothelial cells in culture were incubated with chrysotile (12001295) or crocidolite (12001284) asbestos in efforts to determine whether relatively low amounts of fibers could directly activate endothelial cells. Concentrations of chrysotile and crocidolite which were noncytolytic caused changes in cell morphology, resulting in activation of endothelial cells to a vacuolated, spindle shaped morphology at sites of fiber deposition. Chrysotile fibers caused a time dependent two to four fold increase in neutrophil (PMN) adherence localized to spindled endothelial cells in close contact with fibers. The PMN adherence was inhibited by monoclonal antibodies against intercellular adhesion molecules (ICAM). A 2.5 fold increase in steady state levels of ICAM-1 mRNA in cells exposed to chrysotile asbestos for 8 hours was demonstrated. Similar changes in cell morphology and increases in PMN binding to endothelial cells were demonstrated on exposure to crocidolite asbestos. The authors conclude that exposure to chrysotile or crocidolite asbestos changes endothelial cell morphology and increases PMN adherence.
NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders; Cell-cultures; Cell-damage; Cytotoxic-effects; Asbestos-fibers; Antibody-response; Mammalian-cells; In-vitro-studies
Pharmacology and Toxicology Dartmouth Medical School Remsen 7650 Hanover, NH 03755-3835
12001-29-5; 12001-28-4; 1332-21-4
Toxicology and Applied Pharmacology
Dartmouth College, Hanover, New Hampshire