NIOSHTIC-2 Publications Search

Increased neutrophil adherence to endothelial cells exposed to asbestos.

Treadwell-MD; Mossman-BT; Barchowsky-A

Toxicol Appl Pharmacol 1996 Jul; 139(1):62-70

http://dx.doi.org/10.1006/taap.1996.0143

00233449

Porcine aortic endothelial cells in culture were incubated with chrysotile (12001295) or crocidolite (12001284) asbestos in efforts to determine whether relatively low amounts of fibers could directly activate endothelial cells. Concentrations of chrysotile and crocidolite which were noncytolytic caused changes in cell morphology, resulting in activation of endothelial cells to a vacuolated, spindle shaped morphology at sites of fiber deposition. Chrysotile fibers caused a time dependent two to four fold increase in neutrophil (PMN) adherence localized to spindled endothelial cells in close contact with fibers. The PMN adherence was inhibited by monoclonal antibodies against intercellular adhesion molecules (ICAM). A 2.5 fold increase in steady state levels of ICAM-1 mRNA in cells exposed to chrysotile asbestos for 8 hours was demonstrated. Similar changes in cell morphology and increases in PMN binding to endothelial cells were demonstrated on exposure to crocidolite asbestos. The authors conclude that exposure to chrysotile or crocidolite asbestos changes endothelial cell morphology and increases PMN adherence.

NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders; Cell-cultures; Cell-damage; Cytotoxic-effects; Asbestos-fibers; Antibody-response; Mammalian-cells; In-vitro-studies

Pharmacology and Toxicology Dartmouth Medical School Remsen 7650 Hanover, NH 03755-3835

TXAPA9

12001-29-5; 12001-28-4; 1332-21-4

19960701

Journal Article

76183

Grant

1996

Grant-Number-R03-OH-03267

1

0041-008X

Pulmonary-system-disorders

Toxicology and Applied Pharmacology

NH

Dartmouth College, Hanover, New Hampshire