Quantitation of blood protein adducts of acrolein by tritiated sodium borohydride reduction.
Thakore-KN; Gan-JC; Oandasan-A; Ansari-GA
Toxicol Methods 1992 Jan; 2(4):295-305
A more rapid and sensitive method for estimating acrolein (107028) adducts of plasma albumin and hemoglobin or red cells was developed. Development of such a method would allow the use of a dosimeter for exposure to any active aldehydes including acrolein and crotonaldehyde (4170303). Male Sprague-Dawley-rats were used in the study. A dose dependent increase was not observed in the carbonyl content with increasing concentrations of acrolein. The lowest concentration of acrolein used to quantitate the adduct formation was 0.025 millimolar at an albumin concentration of 1mg/ml. When the commercially purified human hemoglobin was incubated with acrolein, the covalent binding increased with increases in acrolein concentration. The carbonyl content of total plasma proteins and affinity purified albumin increased linearly with increasing acrolein concentrations in the incubation mixture. When whole blood was incubated with acrolein, the total plasma proteins and hemoglobin also showed a dose dependent increase in covalent binding. The authors indicate that the efficacy of this method will need to be established in humans prior to using it for molecular dosimetry.
NIOSH-Grant; Grants-other; Analytical-methods; Chemical-analysis; Analytical-chemistry; Biological-monitoring; Blood-analysis; Protein-chemistry
Human Biol Chem and Genetics University of Texas Med BR Dept of Human Biol Chem&gene Galveston, Tex 77550-2774
Other Occupational Concerns; Grants-other
University of Texas Medical Branch, Galveston, Texas