Taurine content of isolated rat alveolar type I cells.
Banks MA; Porter DW; Pailes WH; Schwegler-Berry D; Martin WG; Castranova V
Comp Biochem Physiol B Comp Biochem 1991 Oct-Dec; 100(4):795-799
The taurine content of rat alveolar type-I cells was compared to that found in alveolar macrophages and type-II cells, and a correlation was made between intracellular taurine levels and susceptibility to oxidant induced injury. The type-I cells were isolated by enzymatic digestion and purified by centrifugal elutriation and specific surface absorption. Sprague-Dawley-rats were used for the study. Taurine levels in alveolar macrophages ranged from 3.86 to 4.68 millimolar (mM), in alveolar type-II epithelial cells from 13.45 to 15.35mM, and in alveolar type-I epithelial cells from 0.07 to 0.21mM, approaching that found in rat plasma, 0.1mM. The authors suggest that type-I cells lack the specialized transport pathway for the active accumulation of taurine. The correlation noted between the rate of oxygen metabolism and intracellular taurine content may involve calcium homeostasis. Cells which are low in taurine would be expected to have low rates of oxygen consumption. The authors conclude that taurine may possess antioxidant properties which are protective to type-II cells and macrophages.
NIOSH-Author; Lung-cells; Cytotoxic-effects; Laboratory-animals; Cell-damage; Oxidation; Antioxidants; In-vitro-studies
V. Castranova, Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505
Comparative Biochemistry and Physiology, Part B: Comparative Biochemistry