Stimulation of alveolar macrophage interleukin-1 production by organic dust-derived products.
Milanowski-J; Sorenson-WG; Lewis-DM; Dutkiewicz-J
Agricultural health and safety: workplace, environment, sustainability, supplement. McDuffie HH, Dosman JA, Semchuk KM, Olenchock SA, Senthilselvan A, eds. Saskatoon, Saskatchewan, Canada: University of Saskatchewan, 1994 Oct; :67-74
A study was done to evaluate the effects of various microbial agents, which have been associated with organic dusts, on macrophage interleukin-1 (IL-1) production. Alveolar macrophages (AMs) were obtained from English-short-hair-guinea-pigs. Freshly isolated AMs at a concentration of 10(7) cells/milliliter were incubated with chromium-51 (Cr51) labeled sodium-chromate. Following exposure to most microbial extracts, the release of Cr51 was concentration dependent. At concentrations of less than 1 microgram/milliliter (microg/ml) the release of Cr51 was no different from that observed in control cells. Concentrations higher than 100microg/ml caused significant leakage of Cr51 within 16 hours. IL-1 production assay results indicated that IL-1 production occurred only following the stimulation of AMs by extracts of Enterobacter-agglomerans and Thermoactinomyces-vulgaris and endotoxin from E-agglomerans. The release of IL-1 was dependent on the dose with maximal effects being obtained at concentrations of 10microg/ml. The findings indicated that endotoxin preparations from E-agglomerans and the extract of E- agglomerans were more potent than Escherichia-coli 055:B5 endotoxin. Higher levels of IL-1 were noted when supernates were tested at a 1:2 dilution, suggesting that inhibitors may have been found in the undiluted supernates. The authors conclude that IL-1 production may be important in the pathogenesis of organic dust induced lung disease.
Lung-disease; Respiratory-system-disorders; Organic-dusts; Dust-inhalation; Dust-exposure; Airborne-particles; Microorganisms; Plant-dusts; Cotton-dust
McDuffie-HH; Dosman-JA; Semchuk-KM; Olenchock-SA; Senthilselvan-A
Agricultural health and safety: workplace, environment, sustainability, supplement