Properties of lavage material from excised lungs ventilated at different temperatures.
Miles-PR; Bowman-L; Frazer-DG
Respir Physiol 1995 Jul; 101(1):99-108
The effects of ventilation temperature on properties of pulmonary lavage material were studied in excised lungs. Lavage from freshly excised rat lungs and excised lungs which had been ventilated at 22, 37, and 42 degrees-C were examined for phospholipid and protein contents, composition of phospholipids, and some surface properties. No differences in the phospholipid composition or surface properties of lavage materials were seen between freshly excised lungs or lungs ventilated at 22 degrees, although slightly more lavageable phospholipids and protein was obtained in the ventilated lungs. Increasing the ventilation temperature of the excised lungs decreased lavage fluid phospholipid content and the relative amounts of total phosphatidylcholine and disaturated phosphatidylcholines and increased unsaturated phosphatidylcholines and total protein and nonsedimentable protein. Lavage material from lungs ventilated at 37 degrees had increased maximum surface tension and those ventilated at 42 degrees had increased maximum and minimum surface tension values. The authors conclude that ventilation temperature affects the composition and surface properties of alveolar lavage materials from excised lungs.
NIOSH-Author; Temperature-effects; Surfactants; Surface-properties; Chemical-composition; Pulmonary-system; Laboratory-animals;
Author Keywords: Mammals; rat; Surfactant; pulmonary; composition; Temperature; pulmonary surfactant
P.R. Miles, Division of Respiratory Disease Studies Appalachian Laboratory for Occupational Safety and Health Morgantown, Morgantown, WV 26505, USA