Generation of reactive oxygen species by quartz particles and its implication for cellular damage.
Shi-X; Mao-Y; Daniel-LN; Saffiotti-U; Dalal-NS; Vallyathan-V
Appl Occup Environ Hyg 1995 Dec; 10(12):1138-1144
The oxygen species generating potential and genotoxic effects of freshly fractured quartz (14808607) were examined. Wet analytical chemistry and electron spin resonance trapping analyses demonstrated the generation of hydrogen-peroxide (7722841), hydroxyl radicals, superoxide radicals, and singlet oxygen (7782447) by freshly fractured quartz particles. The degree of hydroxyl radical generation was inhibited by superoxide-dismutase, catalase, and deferoxamine, and enhanced by hydrogen-peroxide. The freshly fractured quartz was also found to consume molecular oxygen and induce lipid peroxidation in a dose dependent fashion. Lipid peroxidation was inhibited by superoxide-dismutase, catalase, and sodium-benzoate (532321). Freshly fractured silica particles induced breaks when incubated with double stranded DNA. This result did not occur when the incubation was conducted in the absence of oxygen or in the presence of catalase. The authors conclude that freshly fractured quartz particles appear to generate reactive oxygen species that may play a role in quartz induced cellular injury.
NIOSH-Author; NIOSH-Cooperative-Agreement; Quartz-dust; Silica-dusts; Free-radicals; Oxidative-processes; Genotoxic-effects; Pathogenesis
Xianglin Shi, Laboratory of Experimental Pathology, National Cancer Institute, Building 41, Room C301, Bethesda, Maryland 20892
14808-60-7; 7722-84-1; 7782-44-7; 532-32-1
Applied Occupational and Environmental Hygiene
Center to Protect Workers' Rights