Primary contribution of the injector to carryover of a trace analyte in high-performance liquid chromatography.
J Chromatogr A 1993 Feb; 631(1/2):161-163
A study was undertaken to determine the extent to which the injector contributes to the problem of analyte carryover in high performance liquid chromatography (HPLC). Two experiments were conducted to measure a low nanogram amount of N1,N3-bis(pentafluorobenzyl)-N7-(2- (pentafluorobenzyloxy)ethyl)xanthine using HPLC. Despite immediate washing of the injector and HPLC column, analyte carryover was seen after injection of the pure mobile phase. Possible mechanisms for analyte retention in the injector were suspected to be active sites and/or solvent dead volumes. Comparable carryover was seen in trials where gradients were conducted immediately following installation of the clean injector, indicating that the isocratic washing procedure was as effective as gradient conduction. Total carryover in all trials remained below 1% of the originally injected analyte. From this study the authors conclude that the use of two injectors each dedicated to different analyte levels can alleviate the problem of injector induced analyte carryover in HPLC.
NIOSH-Grant; NIOSH-Publication; Grants-other; Analytical-processes; Analytical-methods; Trace-analysis; Chromatographic-analysis; Gas-liquid-chromatography; Sample-preparation
Medicinal Chemistry Northeastern University 360 Huntington Avenue Boston, MA 02115
Other Occupational Concerns; Grants-other
Journal of Chromatography A
Northeastern University, Boston, Massachusetts