Interlaboratory comparison of non-fibrous particle lung burden quantification techniques.
Abraham-JL; Stettler-LE; Burnett-B; Hunt-A; Platek-SF
Ann Occup Hyg, Inhaled Particles VII 1994 Jan; 38(Suppl 1):541-550
An investigation was undertaken to determine the effectiveness of three different approaches to the use of scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) methods for the quantification of lung particle burden. Ten previously examined lung tissue samples were used in this study. Samples were analyzed using a manual in-situ method with 5 micrometer tissue sections (SUNY-in-situ), manual analysis of particles extracted via tissue digestion (SUNY-digestion), and automated techniques on ashed tissue (NIOSH). All three analytical approaches revealed the same profile of particle types, though the methods did not yield consistent results in absolute particle concentrations. Strong Spearman rank order correlations were seen between the SUNY-in-situ and SUNY- digestion methods, and between the SUNY-digestion and NIOSH methods. From this study the authors conclude that discrepancies in the results obtained using the different analytical approaches are possibly attributable to differences in sample preparation methods and variations in minimum size thresholds.
NIOSH-Author; Analytical-methods; Microscopic-analysis; Lung-tissue; X-ray-analysis; Lung-burden; Particulates; Quantitative-analysis
Annals of Occupational Hygiene, Inhaled Particles VII